| 质粒名称: | amilCP_Orange chromoprotein |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 117850 |
| 存储实验室: | Anthony Forster |
|
载体骨架基本信息 |
|
| 载体名称: | BioBrick pSB1C3 vector |
| 载体出品公司: | iGEM Registry |
| 载体修饰: | BioBrick sites removed |
| 载体类型: | 合成生物学；大肠杆菌 |
| 载体抗性: | 氯霉素，25μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | promoter, RBS, amilCP_Orange |
| 物种来源: | Synthetic |
| 插入大小: | 951 bp |
| 基因突变: | 64V,65G, BioBrick sites removed |
|
文献引用方法 |
|
| 材料和方法部分: | amilCP_Orange chromoprotein was a gift from Anthony Forster (Addgene plasmid # 117850 ; http://n2t.net/addgene:117850 ; RRID:Addgene_117850) |
| 参考文献部分: | Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen A, Lundin ER, Wistrand-Yuen E, Forster AC. J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. eCollection 2018. 100 [pii] PubMed 29760772 |
买家导航
amilCP_Orange chromoprotein载体质粒基本信息
amilCP_Orange chromoprotein质粒图谱载体图谱和amilCP_Orange chromoprotein载体序列质粒序列多克隆位点信息
amilCP_Orange chromoprotein质粒载体简介
amilCP_Orange chromoprotein质粒序列载体序列
LOCUS amilCP_Orange chromoprotein 2800 bp DNA circular SYN 15-DEC-2025
DEFINITION BioBrick pSB1C3 plasmid that constitutively overexpresses
amilCP_Orange chromoprotein in E. coli.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2800)
AUTHORS Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen
P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen
A, Lundin ER, Wistrand-Yuen E, Forster AC
TITLE Engineering a palette of eukaryotic chromoproteins for bacterial
synthetic biology.
JOURNAL J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0.
eCollection 2018.
PUBMED 29760772
REFERENCE 2 (bases 1 to 2800)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J Biol
Eng."; date: "2018-05-10"; volume: "12:8. doi"; pages: "
10.1186/s13036-018-0100-0. eCollection 2018"
COMMENT Sequence Label: amilCP_Orange chromoprotein
FEATURES Location/Qualifiers
source 1..2800
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator complement(1..44)
/label=bacterial terminator
/note="putative bacterial transcription terminator"
misc_feature 47..68
/label=BioBrick prefix
/note="BioBrick prefix for parts that do not start with
""ATG"""
promoter 69..103
/label=BBa_J23110
/note="Anderson constitutive promoter
(https://parts.igem.org/Promoters/Catalog/Anderson).
Measured strength relative to BBa_J223100: 0.33"
RBS 112..123
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
misc_feature 799..819
/label=BioBrick suffix
/note="universal suffix for all parts"
terminator 820..877
/label=his operon terminator
/note="This putative transcriptin terminator from the E.
coli his operon has a 2-bp deletion introduced during
synthesis. Its efficiency has not been determined."
primer_bind complement(901..918)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1072..1660)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1152..1171)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
terminator complement(1843..1937)
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(1958..2617)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind 2532..2551
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
primer_bind 2779..2797
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
61 ttctagagtt tacggctagc tcagtcctag gtacaatgct agctactaga gaaagaggag
121 aaatactaga tgagtgtgat cgctaaacaa atgacctaca aggtttatat gtcaggcacg
181 gtcaatggac actactttga ggtcgaaggc gatggaaaag gtaagcccta cgagggggag
241 cagacggtaa agctcactgt caccaagggc ggacctctgc catttgcttg ggatatttta
301 tcaccacagg ttggatacgg aagcatacca ttcaccaagt accctgaaga catccctgac
361 tatgtaaagc agtcattccc ggagggctat acatgggaga ggatcatgaa ctttgaagat
421 ggtgcagtgt gtactgtcag caatgattcc agcatccaag gcaactgttt catctaccat
481 gtcaagttct ctggtttgaa ctttcctccc aatggacctg tcatgcagaa gaagacacag
541 ggctgggaac ccaacactga gcgtctcttt gcacgagatg gaatgctgct aggaaacaac
601 tttatggctc tgaagttaga aggaggcggt cactatttgt gtgaatttaa aactacttac
661 aaggcaaaga agcctgtgaa gatgccaggg tatcactatg ttgaccgcaa actggatgta
721 accaatcaca acaaggatta cacttcggtt gagcagtgtg aaatttccat tgcacgcaaa
781 cctgtggtcg cctaataata ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt
841 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc
901 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
961 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
1021 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag
1081 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
1141 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
1201 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
1261 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
1321 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
1381 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
1441 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
1501 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
1561 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
1621 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
1681 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
1741 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
1801 aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa
1861 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
1921 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat
1981 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
2041 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
2101 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
2161 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
2221 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
2281 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
2341 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa
2401 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
2461 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
2521 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
2581 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
2641 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
2701 aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc
2761 atgacattaa cctataaaaa taggcgtatc acgaggcaga
//
